Author Correction: CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies
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چکیده
منابع مشابه
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies
Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11...
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Background: It is now well-demonstrated that histone demethylases play an important role in developmental controls, cell-fate decisions, and a variety of diseases such as cancer. Lysine-specific demethylase 5D (KDM5D) is a male-specific histone demethylase that specifically demethylates di- and tri-methyl H3K4 at the start site of active genes. In this light, the aim of this study was to invest...
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18 Connexins (Cx) form gap junction channels made up of two connexons 19 (hemichannels) from adjacent cells. Unopposed hemichannels may open towards the extracellular 20 space upon stimulation by e.g. removal of divalent cations from the extracellular solution and allow 21 isoform-specific transmembrane flux of fluorescent dyes and physiologically relevant molecules, 22 such as ATP and ions. Cx...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2021
ISSN: 1474-760X
DOI: 10.1186/s13059-021-02314-1